characteristic

SYQX—Ⅱ/Type IIIAn anaerobic incubator consists of a vacuum sampling chamber, a constant temperature anaerobic operating chamber, an air circuit, an electrical control system, and other components. The whole machine has a novel design, compact structure, good anaerobic environment, good air tightness performance, high temperature control accuracy, good stability, convenient use, gas saving, economy, safe and reliable operation, and other advantages. Its characteristics are as follows:

1. Using advanced scientific methods to achieve high precision in anaerobic environments, with good constant performance and reliable use;

2. The temperature of the incubator is controlled by a microcomputer, which can accurately and intuitively reflect the temperature inside the incubator, and can automatically control the temperature again. It is an effective temperature limiting protection device to ensure that the culture grows under safe temperature conditions;

3. The box is equipped with ultraviolet sterilization lamps, which can effectively prevent bacterial contamination;

4. Gas path device, capable of accurately adjusting flow rate and inputting various required gases at will;

5. The pneumatic circuit is controlled by a microcomputer, and the solenoid valve can be started or closed with flexible operation;

6. The front window of the operating room is made of thick transparent special glass, which allows for clear and direct observation of indoor operations. The use of plastic gloves is reliable, comfortable, flexible, and easy to use;

7. There is a deoxygenation catalyst installed indoors.

III Specification

1. The anaerobic state formed in the sampling room takes less than5minute;

2. The anaerobic formation time in the operating room is less than1Hour (pumping and inflation replacement);

3. When the operation room stops replenishing trace amounts of mixed gas, the anaerobic environment is maintained for a longer period of time than10Hour;

4. Operating temperature: room temperature+3℃~50℃；

5. Temperature fluctuation in the cultivation room ≤±0.3℃； Temperature distribution uniformity ≤±1.0℃；

6. Operating room volume:710mm×530mm×590mm(length x height x width),

7. power supply220V±10%AC, Power:500VA

4 install

1. The whole machine should be placed in a location with a small temperature difference and easy operation, avoiding direct sunlight and staying away from heating equipment, and placed steadily;

2. Place the mixed gas cylinder and nitrogen cylinder steadily, and install pressure reducing valves (including pressure gauges) in appropriate positions;

3. Connect the air circuit and check it. To prevent air leakage, if necessary, use sealant to bond at each connection point;

5 use

（1） Formation of anaerobic environment in the operating room:

1. Place necessary accessories and tools according to usage requirements;

2. Turn on the power, turn on the lighting, turn on the temperature controller, and adjust the desired temperature;

3. Placed in the operating room1000gPalladium particles (closed) and500gDesiccant and put it in Meilan indicator paper (sealed);

4. Close the indoor and outdoor doors of the sampling room tightly and perform vacuum calibration;

5. Number of replacements in the operating room (nitrogen replacement):

A. Put latex gloves on the flange ring of the observation board and tighten them tightly

B. Connect the nitrogen intake path and open the nitrogen control valve1Make the gloves bulge and close the valve1Then tighten the bag tightly;

6. Repeat the nitrogen filling process for the second replacement of the operating room (nitrogen replacement), evacuate the sampling room first, and pay attention to using the foot switch to open and close the exhaust at any time. Three consecutive nitrogen replacements.

7. The fourth replacement of the operating room (mixed gas replacement):

The ratio of mixed gases isN₂ 85%、H₂ 10%、CO₂ 5%）

A. Swap the air circuit and open the mixing valve3During intake and inflation, the sampling chamber should be evacuated first, and the exhaust should be opened and closed at any time using a foot switch;

B. Close the mixed gas valve3And open the valve5Pass the mixed gas through the flow meter, adjust the flow meter to a flow rate of per minute10mlabout;

C. Repetition of mixed gas2-3Through the above process of secondary conversion, an anaerobic environment is basically formed.

8. Turn on the granular deoxygenation agent in the operating room, connect the power supply of the deoxygenation catalyst for catalytic deoxygenation, and after one hour, open the Meilan indicator paper to observe its color change. If it does not change color, it means that the operating room has reached an anaerobic environment;

9. Turn on the ultraviolet sterilization lamp and sterilize indoors. The sterilization time is determined according to the specific experiment.

（2） Placement and cultivation of bacterial strains:

1. Check the door inside the sampling room and close it tightly;

2. Open the outdoor door of the sampling room, place the bacterial strain into the sampling room, and then close the door;

3. Three rounds of nitrogen replacement process in the sampling chamber: turn on the vacuum pump and first evacuate the vacuum level500mlMercury column（66kPa）Stop above and manually open the nitrogen valve2Air intake, reset the pointer to zero position, and close the valve2Repeat the operation once for the second time. During the third operation, increase the vacuum level500mlMercury column（66kPa）Stop above, then open the valve4Inject the mixed gas, make the pointer return to zero position, and close the valve4The three nitrogen replacement processes in the sampling room have been completed;

4. If a lower vacuum degree is selected, the number of replacements needs to be increased;

5. Open and close the outdoor door for sampling, then lower the vacuum level100mlMercury column（13kPa）Inspection;

6. Conditions for long-term continuous use of anaerobic incubators:

A. Open the Meilan indicator paper in the operating room every day to observe. If it is not normal, it must be ventilated again;

B. Long term continuous input of trace amounts of mixed gas is required to enable the nitrogen gas to combine with trace amounts of oxygen through catalytic absorption, ensuring anaerobic conditions indoors. The flow rate of the mixed gas is selected as per minute10mlabout;

C. Continuously cultivate and run for one day, and replace the deaerator and desiccant once.

D. The temperature inside the operating room can be freely selected and controlled.

7. Adjustment of output pressure for mixed gas cylinders and nitrogen cylinders: Adjust the pressure reducing valve to increase the output pressure0.1Mpaabout.

VI precautions

1. Instruments should be installed in places with clean air and minimal temperature changes as much as possible;

2. Before starting up, it is necessary to fully familiarize and understand the instructions for using the various components and supporting instruments, and master the correct usage methods;

3. The culture must be placed after reaching an absolute anaerobic environment in the operating room;

4. In the event of a malfunction (such as gas stoppage), the operating room can still be maintained10Hour anaerobic state (exceeding10If needed, the culture should be taken out for further processing;

5. Regularly pay attention to whether there is any air leakage in the air circuit;

6. When replacing gas cylinders, be careful to tighten the trachea to avoid the flow of oxygen-containing gas;

Use the vacuum pump as required and regularly check and refuel it.