PlantScreen-SCPlant Phenotype Analysis and Measurement System (Desktop)

PlantScreen SC is a mini version of the high-throughput PlantScreen plant phenotype analysis platform (also known as the Bench top version). RGB imaging, chlorophyll fluorescence and other imaging sensors are configured in a compact box, and plant samples are manually placed inside the box for imaging analysis (instead of automatic conveyor belts). It has the advantages of simple and compact structure, easy installation and use (generally applicable in laboratories or greenhouses), high cost-effectiveness (saving expensive and complex conveyor belt systems or XYZ 3D mobile robotic arm systems), and comprehensive functions.

The PlantScreen SC plant phenotype imaging analysis platform is equipped with RGB visible light color 3D imaging analysis technology and chlorophyll fluorescence technology for plants. Suitable for laboratory or greenhouse crop phenotype imaging analysis research, the cultivated plants are manually placed on the sample stage in the system (manual sample loading), and the sample stage can rotate 360 degrees for imaging analysis of plants from different angles. It is possible to obtain plant growth morphology and structural parameters, color analysis, growth and development, and physiological function parameters of plants and fruits at different growth stages, such as leaf morphology measurement, growth rate, plant growth structure, chlorophyll fluorescence, photosynthetic system operation mechanism, pigment composition, stress and resistance, etc., to achieve rapid, efficient, and non-destructive measurement and analysis of crop phenotype and physiological ecology, thereby screening out plant materials with excellent plant behavior and strong stress resistance, accelerating the process of crop breeding, and is an important equipment for genetic genomics, phenotype genomics, genetic breeding and other research.

The main functional features are as follows:

1) RGB 3D imaging analysis: including high-resolution color imaging of the top and sides, and equipped with a 360 ° rotating platform, it can perform three-dimensional morphological structure and color analysis and measurement of plants. It can be used for morphological structure and color analysis of plants, fruits, seeds, and growth and development analysis, including size (width, length, area, etc.), compactness, symmetry (eccentricity, roundness, centroid, etc.), color analysis (greenness index, leaf lesions, fruit ripening process, fungal infection process analysis, different color area ratios, etc.). Through the chlorophyll fluorescence monitoring module, it is possible to further measure and analyze the photosynthetic efficiency, fitness, biotic and abiotic stress levels and sensitivity of plants, as well as their stress resistance (such as disease resistance, drought resistance, etc.).

The chlorophyll fluorescence monitoring unit uses unmanned automatic monitoring of real-time chlorophyll fluorescence Ft, photon yield QY, Kautsky induction effect, fluorescence quenching, light response curve, and OJIP rapid fluorescence kinetics curve measurement protocol to monitor physiological traits such as plant photosynthetic efficiency, fitness, light energy utilization efficiency, stress and resistance. It is used for plant physiological and ecological functions, plant stress physiology research, and resistance screening.Integrated advanced chlorophyll fluorescence technologies such as PAM (pulse modulation technology) fluorescence measurement technology and OJIP rapid fluorescence kinetics measurement technology. More than 50 chlorophyll fluorescence parameters can be obtained, including F0, Ft, Fm, Fm ', QY, QY1 Ln, QY1 Dn, NPQ, Qp, Rfd, PAR, Area, Mo, Sm, PI, ABS/RC, etc.

2) Environmental factor monitoring, synchronous monitoring of temperature, humidity, photosynthetically active radiation and other environmental factors

3) PlantScreen phenotype big data analysis platform

Technical parameters:

1. Host system;

1.1 Intelligent LED cold white light source

1.2 Built in temperature, humidity, and light intensity sensors for synchronous monitoring of environmental factors

1.3 360 degree rotating platform

1.4 PlantScreen Phenotype Server, PlantScreen Program Control System (Scheduler)

1.5 GUI (graphical user interface) experimental setup and control, real-time display of system operating status

1.6 Support multi-user registration, personalized experiment planning, management, storage, display, and analysis

1.7 Networking: All network components comply with the 1GBit standard, and the main router provides DHCP and routing services for the system intranet. It can also be connected to vendor servers for system diagnosis, software updates, etc

1.8 Main computer: Ryzen 5 3600 processor, 8GB RAM, 256GB SSD solid state drive, 6TB RAID5 storage, Windows 7 Pro operating system, matching display.

2 RGB 3D imaging analysis:

2.1 5MP CMOS RGB imaging lens, sensor size 1/2 "

2.2 Resolution 2560x1920 pixels, pixel size 2.2 μ m; Frame rate: 14fps@5MP

2.3 Optical lens: 8mm SV0814H

2.4 Adjustable imaging height to adapt to plants of different heights

2.5 includes two imaging lenses on the top and side, as well as a rotating platform (for controlling plant samples), which can measure and analyze images from various angles

2.6 It can measure leaf area, circumference, width, and other morphological measurements and growth analysis, and can perform color segmentation analysis, etc

3. Chlorophyll fluorescence monitoring:

3.1 Integrated advanced chlorophyll fluorescence technologies such as PAM (pulse modulation technology) fluorescence measurement technology and OJIP rapid fluorescence kinetics measurement technology

3.2 Protocols: Can automatically run real-time chlorophyll fluorescence Ft, photon yield QY, Kautsky induction effect, 2 sets of fluorescence quenching, 3 sets of photoresponse curves, and nearly 10 measurement program protocols such as OJIP test

3.3 More than 50 measurement parameters were measured, among which OJIP test recorded nearly multiple chlorophyll fluorescence data within 1 second and calculated 26 chlorophyll fluorescence parameters including PI (Performance Index) through software

3.3 Measurement light 0-100% adjustable, pulse modulation 0.01-0.09 µ mol.m-2.s-1; Photochemical light 10-1000 µ mol.m-2.s-1 adjustable, saturated light pulse 10-100% adjustable (up to 3000 µ mol.m-2.s-1)

3.4 Data Communication: USB or Bluetooth

3.5 operation keys and LC display screen

4. Morphological analysis measurement parameters:

4.1 Leaf area

4.2 Leaf circumference

4.3 Eccentricity

4.4 Roundness

4.5 circle diameter

4.6 Medium Leaf Width Index

4.7 Solidity/compactness

4.8 Convex hull area

4.9 Centroid

4.10 Various morphological and structural parameters such as leaf or fruit length and width

4.11 * Time series analysis to analyze the growth rate of plant leaves and fruits

4.12 Comparative analysis of leaf area during the entire growth or experimental period, etc

5 Color segmentation analysis and measurement

5.1 Greenness index, etc

5.2 Different color area ratios, etc

5.3 Color segmentation for plant suitability evaluation, etc

5.4 Analysis of color development changes in leaves or fruits, including changes in morphological and color development processes

5.5 Color Cluster Analysis

6 Chlorophyll fluorescence measurement parameters

6.1 Measurement parameters include F0 Ft、Fm、Fm’、QY、QY_Ln、QY_Dn、NPQ、Qp、Rfd、PAR、Area、Mo、Sm、PI、ABS/RC Waiting for more than 50 chlorophyll fluorescence parameters, as well as light response curves for 3 light application programs, 2 fluorescence quenching curves, OJIP curves, etc

6.2. OJIP - Test with a time resolution of 10 µ s (100000 times per second), providing the OJIP curve and 26 parameters, including F0 Fj、Fi、Fm、Fv、Vj、Vi、Fm/F0、Fv/F0、Fv/Fm、Mo、Area、Fix Area、Sm、Ss、N、Phi_Po、Psi_o、Phi_Eo、Phi–Do、Phi_Pav、PI_Abs、ABS/RC、TRo/RC、ETo/RC、DIo/RC etc.

6.3 Built in automatic running protocols: Ft, QY, OJIP, NPQ1, NPQ2, LC1, LC2, LC3, etc

6.4 The system has three operation modes: automatic mode, manual mode, and server mode. The automatic mode automatically runs the set program, and data is automatically processed and stored in the database; Manual mode starts the program manually; Server mode is used for system calibration, snapshots, sensor detection, error reporting, etc

7 phenotype analysis big data platform (servers, etc.)

7.1 Phenotype Analysis System (Plant DATA Analyzer), Application Server, Database, etc

7.2 Online visualization displays the experimental process, results, etc

form:

Main chassis; Phenotype analysis big data platform, including system control communication software package, data analysis, server, etc., used for system control, data collection and analysis, etc; Three dimensional RGB imaging unit, including two RGB lenses on the top and side, and a rotating plant sample rack; Chlorophyll fluorescence measurement unit

Origin: Czech Republic