PlantScreen Plant Phenotype Imaging Analysis System (XYZ 3D Mobile Imaging Version)

PlantScreenThe plant phenotype imaging system is developed and produced by PSI company in the Czech Republic,Integrated with advanced technologies such as Robotic automation control system, intelligent LED lighting cultivation system, chlorophyll fluorescence imaging measurement and analysis, plant thermal imaging analysis, plant near-infrared imaging analysis, plant hyperspectral analysis, automatic barcode recognition management, RGB true color imaging, etc,To achieve comprehensive physiological, ecological, and morphological imaging analysis of a large number of plant samples in an optimized manner, from Arabidopsis thaliana, corn to various other plants, for high-throughput plant phenotype imaging analysis, plant stress response imaging analysis, plant growth analysis, ecological toxicology research, trait identification, and plant physiological and ecological analysis research. As the world's first manufacturer to develop and produce plant chlorophyll fluorescence imaging systems, PSI company is at the forefront of technology in the field of plant phenotype imaging analysis. The large-scale chlorophyll fluorescence imaging analysis function of PlantScreen enables it toBecoming the most advanced instrument and equipment for plant phenotype analysis and functional imaging analysis, enabling over 100 measurement parameters such as plant growth and stress response.

PlantScreenThe system includes the following imaging analysis functions:

1.Large scale chlorophyll fluorescence imaging analysis: single image area of 35x35cm, imaging measurement parameters include Fo, Dozens of chlorophyll fluorescence parameters including Fm, Fv, Fo ', Fm', Fv ', Ft, Fv/Fm, Fv'/Fm ', Phi-PSII, NPQ, qN, qP, Rfd, etc

2.RGBImaging analysis: Imaging measurement parameters include:

1)Leaf Area: Useful for monitoring growth rate）

2)Plant solidity/compactness. Ratio between the area covered by the plant’s convex hull and the area covered by the actual plant）

3)Leaf circumference: Particularly useful for the basic leaf shape and width evaluation (combined with leaf area)）

4)Eccentricity: Plant shape estimation, scalar number, eccentricity of the ellipse with same second moments as the plant (0...circle, 1...line segment)）

5)Roundness of leaves: Based on evaluating the ratio between leaf area and perimeter. Gives information about leaf roundness）

6) Medium Leaf Width Index: Leaf area proportional to the plant skeleton (i.e. reduction of the leaf to line segment)）

7)Slenderness of Leaves (SOL)

8)Plant Circle Diameter. Diameter of a circle with the same area as the plant）

9)Convex Hull Area. Useful for compactness evaluation）

10)Plant Centroid. Center of the plant mass position (particularly useful for the eccentricity evaluation)）

11)Internodal distances

12)Growth Height

13)Maximum Height and Width of Plant in 3 Dimensions

14)Relative growth rate

15)Leaf Angle

16)Leaf Number at Nodes

17)Other parameters such as color segmentation for plant fitness evaluation, greening index and others

3.Hyperspectral imaging analysis (optional), capable of imaging and analyzing the following parameters:

1)Normalized Difference Vegetation Index (NDVI)

2)The Simple Ratio Index,Equation: SR = RNIR / RRED）

3) Improved Chlorophyll Absorption in Reflectance Index (MCARI1),
Equation: MCARI1 = 1.2 * [2.5 * (R790- R670) - 1.3 * (R790- R550)]）

4)Optimized Soil Adjusted Vegetation Index (OSAVI)
, Equation: OSAVI = (1 + 0.16) * (R790- R670) / (R790- R670 + 0.16)）

5)Greenness Index (G),Equation: G = R554 / R677）

6)Improved Chlorophyll Absorption in Reflectance Index (MCARI),
Equation: MCARI = [(R700- R670) - 0.2 * (R700- R550)] * (R700/ R670)）

7)Transformed CAR Index (TCARI)
, Equation: TSARI = 3 * [(R700- R670) - 0.2 * (R700- R550) * (R700/ R670)]）

8)Triangular Vegetation Index (TVI)
,
Equation: TVI = 0.5 * [120 * (R750- R550) - 200 * (R670- R550)]）

9)ZMIZarco Tejada&Miller Index (ZMI),Equation: ZMI = R750 / R710）

10)Simple Ratio Pigment Index (SRPI),Equation: SRPI = R430 / R680）

11)Normalized Phaeophyteinization Index (NPQI),Equation: NPQI = (R415- R435) / (R415+ R435)）

12)Photochemical Reflectance Index (PRI),Equation: PRI = (R531- R570) / (R531+ R570)）

13)Normalized Pigment Chlorophyll Index (NPCI), NPCI = (R680- R430) / (R680+ R430)）

14)CarterCarter Indices
, Equation: Ctr1 = R695 / R420; Ctr2 = R695 / R760）

15)LichtenthalerLichtenhaler Indicators
, Equation: Lic1 = (R790 - R680) / (R790 + R680); Lic2 = R440 / R690）

16)SIPIStructure Intensive Pigment Index (SIPI),Equation: SIPI = (R790- R450) / (R790+ R650)）

17)Gitelson- Merzlyak Index (Gitelson and Merzlyak Indicators)
,
Equation: GM1 = R750/ R550; GM2 = R750/ R700）

4.Thermal imaging analysis (optional): used for imaging analysis of the two-dimensional heat distribution of plants under light radiation conditions. Good heat dissipation can enable plants to tolerate long periods of high light radiation or low water conditions (drought)

5.Near infrared imaging analysis (optional): used to observe and analyze the water status of plants and their distribution variation among different tissues. Plants in good irrigation conditions exhibit high absorption of near-infrared spectra, while plants in drought conditions exhibit high reflectivity of near-infrared spectra. Through analysis software, the entire process from drought stress to re irrigation can be monitored and analyzed dynamically, as well as the plant's response to drought stress and water use efficiency, and false color images can be formed, which can be correlated with the plant's morphological index and chlorophyll fluorescence index for analysis and research.

System configuration and working principle:

PlantScreenXYZ 3D Mobile Imaging Version of Plant Phenotype Imaging Analysis System（PlantScreenThe basic configuration of XYZ Rotetic System includes an automated XYZ 3D operating system, RGB imaging FluorCamChlorophyll fluorescence imaging, etc. can be optionally equipped with hyperspectral imaging, plant thermal imaging, plant near-infrared imaging, plant identification system, etc. The imaging system is moved by an automated 3D robotic arm above the plant for imaging analysis, etc

Technical indicators:

1.XYZThe three-axis robotic arm can freely move above the plant for imaging analysis, and the size can be customized according to customers. The standard configuration of the mobile imaging platform can perform XYZ three-dimensional scanning imaging on four 110x170cm plant cultivation stages. The cultivation stage is 75cm high, and the imaging platform is adjustable from 100cm to 150cm in height (automatic control)

2.Chlorophyll fluorescence imaging: lens resolution 1392x1040 pixels, single frameThe imaging area is 35x35cm, and the measurement light is orange 618nm. The orange and white dual wavelength photochemical light is used, and the saturated light flash is white. The maximum light intensity is 3600 μ mol/m²/s. Equipped with 735nm infrared light source

3.RGBImaging: High sensitivity imaging sensor 1/2.5 ", resolution 2592x1944 pixels, pixel size 2.2 μ m, automatic or manual exposure and white balance, etc,The imaging information includes time and location, and the recording format is date month year hour minute second Pos_X_YZ.bmp

4.RGBImaging analysis and processing: barrel distortion calibration function, tray detection and clipping processing function, background subtraction function

5.NIRNear infrared imaging unit (optional): capable of capturing images in the 1450-1600nm water absorption band to reflect plant moisture status. It exhibits high NIR absorption values under abundant water supply and high NIR reflection under drought stress. NIR false color imaging can reflect and analyze plant moisture status through software

6.Hyperspectral imaging unit (optional), 1000-2500nm (SWIR) lens or 400-1000nm (VNIR) lens, field of view 150x100cm

7.Thermal imaging unit: resolution 640x480 pixels, temperature range 20-120 ° C, sensitivity NETD<0.05 ° C @ 30 ° C/50mK, imaging area up to 150x150cm

8.System Control and Data Acquisition Analysis System: User friendly graphical interface, user-defined and editable automatic measurement programs (protocols), control units with main power switch, emergency shutdown, XYZ 3D axis start switch, pause button, move button, etc., username and password protection

Place of Origin:Europe