0.1%Sirius Red is dissolved in saturated picric acid solution and used for staining connective tissues such as myocardium.

Ordering information:

Recommended staining process for Sirius Red paraffin sections:

1. Deparaffinize and hydrate to distilled water.

2. Wash in running tap water, 10 minutes.

3. Rinse in distilled water.

4. Treat in Phosphomolybdic Acid(Phosphomolybdic acid solution)0.2% Aqueous (1-5 minutes)

5. Stain in Sirius Red, 0.1% in Sat'd Picric Acid , for 90 minutes.

6. Wash for 2 minutes in 0.01 N Hydrochloric Acid .

7. Rinse in 70% Alcohol for 45 seconds.

8. Dehydrate, clear and mount in Permount(Sealing agent)