ORCA-Flash4.0 LT3 is a new scientific CMOS camera for fluorescence imaging, which is an improvement based on the ORCA Flash 4.0 released in 2011. It has the high-level performance required for fluorescence imaging in life science applications, and can be used not only for basic research applications, but also integrated into various types of devices.
characteristic
Low readout noise (standard scan): 1.5 electrons (rms), 0.9 electrons (median)
Fast read (fast scrolling, full resolution): 40 frames per second
Large field of view: 13.312 mm (H) × 13.312 mm (V)
High resolution: 2048 (H) × 2048 (V)
High quantum efficiency: 82% (peak QE)
purpose
Delay imaging
Multidimensional imaging
Ca2+imaging
DNA chip reading
High signal-to-noise ratio comparable to or better than BSI cameras
The following figure compares the readout noise distribution of the second-generation BSI camera *, indicating that ORCA-Flash4.0 LT3 has more pixels and lower readout noise.
ORCA-Flash4.0 LT3 has a low readout noise of 1.5 electronic rms, providing high signal-to-noise ratio comparable to or better than BSI cameras, especially in low light ranges.
This performance can be demonstrated in high-quality multidimensional imaging, such as long delay imaging with low excitation light, low phototoxicity, and photobleaching.
*BSI camera: back illuminated camera
Quickly read out
The ORCA-Flash4.0 LT3 can achieve a read rate of 40 frames per second, even faster than the traditional ORCA-Flash4.0 LT+(30 frames per second). This is very useful for applications that require high temporal resolution for real-time measurement.
VIEW
The wide field of view of 13.312 mm (H) × 13.312 mm (V) supports obtaining more information in a single shot.
This helps to improve the throughput of experiments such as DNA chip reading.
detailed parameters
Product Code | C11440-42U40 |
Imaging equipment | Scientific CMOS Image Sensor |
Effective pixel count | 2048 (H) × 2048 (V) |
Pixel size | 6.5 μ m × 6.5 μ m |
Effective area | 13.312 mm × 13.312 mm |
Maximum trap capacity (typical value) | 30000 electrons |
Dynamic range (typical values) | 33 000:1*¹20 000:1*² |
Cooling method | Forced air cooling, Peltier cooling |
Cooling temperature | +10 ° C (forced air cooling, fan speed: fast, ambient temperature:+ 10 ° C to+30 ° C) |
Frame rate at full resolution | Fast scrolling: 40 frames per second Standard scan: 30 frames per second |
Read out noise (typical value) | Fast scrolling: 1.9 electrons (rms), 1.3 electrons (median) Standard scan: 1.5 electron rms/0.9 electron (median) |
Dark current (typical value) | 0.6 electrons/pixel/second (typical value) (cooling temperature:+ 10 ̊C) |
Digital output | 16 bits |
Exposure time |
Internal trigger mode * ³ (fast scrolling): 1 ms to 10 Internal trigger mode * ³ (standard scan): 3 ms to 10 s Internal trigger mode with subarray readout (fast scrolling): 40 μ s to 10 s Internal trigger mode with subarray readout (standard scan): 129.99 μ s to 10 s |
External trigger input mode | Edge trigger, global reset edge trigger, level trigger, global reset level trigger, synchronous readout trigger, start trigger. |
Trigger delay function | 0 s to 10 s, with a step size of 1 μ s |
Trigger input connector | SMA (SMA) |
Trigger output | 3 programmable timed outputs, trigger ready output, global exposure timed output, low output, high output |
Trigger output connector | SMA (SMA) |
Pixel Merge | 2 × 2, 4 × 4 |
Subarray | Yes, it is |
Interface | USB 3.1 Gen 1 |
Lens interface | C-type interface |
Power supply | AC 100V to AC 240V, 50Hz/60Hz |
Power consumption | About 75 VA |
Operating environment temperature | 0 ° C to+40 ° C (fan speed: fast) |
Operating environment humidity | 30% to 80% (no fog phenomenon) |
Storage environment temperature | -10 ° C to+50 ° C |
Storage environment humidity | Up to 90% (no fog phenomenon) |
*Calculate the median based on the ratio of maximum well capacity to readout noise in standard scanning
*Calculate (rms) based on the ratio of maximum well capacity and readout noise in standard scanning
*The minimum exposure time factor in internal triggering mode varies depending on the size and position of the array.
Spectral sensitivity characteristics
size
Imaging of cells in different dimensions
Cellular multi wavelength synthesis3Drebuild