Royal jelly is in the worldrecognized****Super nutritious food with high active ingredients that can be directly consumed by humans. 10-Hydroxy-2-decenoic acid (10-HDA) is a unique unsaturated fatty acid found in royal jelly,Since this acid is not found in other substances in nature and only exists in royal jelly, it is also known as royal jelly acid. The separated pure royal jelly acid appears as white crystals,utensilHas excellent bactericidal and bacteriostatic propertiesAnti fatigue, anti-agingFunction and anti-cancer, anti radiation properties，For this reason, many health foods are added with royal jelly. Therefore, as one of the main indicators for evaluating the quality of royal jelly and its products, decenoic acid（10-HDA）Content determination is particularly important. So far, there are various methods for determining the content of 10-HDA, including thin-layer scanning, spectrophotometry, gas phase method, and liquid phase method, which are commonly used for analyzing 10-HDA in royal jelly. Thin layer scanning and spectrophotometry have poor specificity and accuracy, while gas phase method is cumbersome to process. Currently, the most commonly used method is HPLC method. The liquid-phase method is simple to process and yields accurate resultsCompany researchersbe based onOptimization of numerous measurement schemes，useLiquid chromatography for analysis of arachidonic acid in royal jellyLC-3000CThe entire systemA method was established for determining the content of decenoic acid 10-HDA in royal jelly and its products by reverse phase liquid chromatography using ethyl p-carboxybenzoate as an internal standard after ultrasonic extraction and refrigeration precipitation, and satisfactory results were obtained.

1Main performance characteristics:
1）、Liquid Chromatography LC-3000C for Analysis of Absolenic Acid in Royal JellyThe entire system is composed of P3000High pressure infusion pump (binary gradient), UV3000 spectrophotometerDetectorAnalytical dynamic high-pressure mixerInjection valveImport, solvent tray, chromatographic columnReverse control chromatographyworkstandSoftware and other components; Installation, debugging, and usage are all very simple.
2）、 The P3000 high-pressure infusion pump is a high-precision infusion pump system controlled by a microcontroller with CPLD technology as the core. The all digital frequency generation system, advanced motor drive system, and user-friendly human-machine display interface make operation very convenient. The advanced pump head and single valve design ideas integrate the world's best experience, ensuring the accuracy and repeatability of infusion under various conditions.

3）、 UV3000 is a spectrophotometer that can be matched with any infusion pump and is widely used in fields such as chemical analysis, pesticides, pharmaceuticals, and chemical engineering. Adopting a fully digital and highly integrated design concept, greatly reducing the noise of the instrument; Based on digital signal transmission and processing, the signal of this detector can be directly input into the serial port of the computer for acquisition, completely eliminating the data acquisition card used by traditional instruments!

2、 Main configuration and testing analysis conditions of the instrument:

(1)Main instrumentsto configure:
1LC-3000 effective liquid chromatography instrumenthost；2. Chromatography column: C18250 * 4.6 * 10 or 250 * 4.6 * 5
3High speed centrifuge(greater than 4500r/min)；50ml）4、Ultrasonic oscillator;5Vortex mixer;
6Analytical balance (one ten thousandth);7Solvent filter (with 0.45um organic and aqueous filter membranes and vacuum pump);
（2) Main reagents:

1.Purified water (secondary distilled water)2.Methanol: Analytical alcohol3.Ethanol: High grade pure4.Internal standard: Methyl p-hydroxybenzoate content 99.0%5.10-HAD standard: above 99.0%. Before use, it should be dried under reduced pressure for 24 hours in a vacuum dryer containing concentrated sulfuric acid610-HAD standard solution: Take approximately 25mg of dried 10-HAD standard, weigh accurately, dissolve in anhydrous ethanol, and transfer to a 1000ml volumetric flask. Dilute to the mark with anhydrous ethanol and shake well. This solution contains approximately 0.65mg of internal standard per milliliter.7.Hydrochloric acid (C=0.03mol/L): Take 100ml of 0.1mol/L hydrochloric acid and add it to 200ml of distilled water.

（3）.Chromatographic conditions:
The chromatographic column isKromasil C18Column, mobile phase isV(methanol):V(water):V(phosphoric acid)=55:45:0.2, flow rate1.0ml/minUsing ethyl carboxybenzoate as an internal standard, detection wavelength210nmThis method is simple, fast, accurate, with good reproducibility and high recovery rate(97.6%～101.8%).

（4）. Sample processing

After thawing the sample to room temperature, stir it evenly with a glass rod, take about 0.5g, and place it in a 50ml volumetric flask that has been weighed accurately. Add 1ml of 0.03mol/L hydrochloric acid and 2ml of water, mix them on a vortex mixer to dissolve the sample, add 30ml of anhydrous ethanol, gently shake while adding, and then add 10ml of internal standard solution precisely. Dilute it to the mark with anhydrous ethanol, shake well, immediately place it in ultrasonic waves for 15 minutes, or shake it on a vortex mixer for 15 minutes, take it out, centrifuge at 3000r/min for 10 minutes, and then measure. If it cannot be measured in a timely manner, it should be stored in the refrigerator for testing.

companyThe clothingaffairAdvantages:
1The companyofafter-sale serviceEngineers have undergone chemical analysis、Training on instrument repair and maintenance，They can maintain and repair various components，To ensure that your instrument runs smoothly and quickly，Can guarantee to solve your problems quickly.
2、technical support----faultarrangeExcept for experts answering your calls，Provide timely solutions to problems.
3、****Service----distributionProvide high-quality services within the scope of * * * *.
4、flexiblefastResponse time after phone repair report, providetimelyGuidance orDoor to door problem-solving.
524 hours non-stopOnline Instrument Problem Answering.
6、There are free and irregular chromatography training courses every year.The content includes chromatographic principles、analysis、application、Technical instrument operation，helpAssist the causeObstacle removal and maintenance.

companyThe clothingaffairAdvantages:
1The companyofafter-sale serviceEngineers have undergone chemical analysis、Training on instrument repair and maintenance，They can maintain and repair various components，To ensure that your instrument runs smoothly and quickly，Can guarantee to solve your problems quickly.
2、Timely telephone support----faultarrangeExcept for experts answering your calls，Provide timely solutions to problems.
3、****Service----distributionProvide high-quality services within the scope of * * * *24 hours non-stopOnline Instrument Problem Answering4、There are free and irregular chromatography training courses every year.The content includes chromatographic principles、analysis、application、Technical instrument operation，helpAssist the causeObstacle removal and maintenance.

Other analysis plans of the company: