Hitachi Ultra High Performance Liquid Chromatography System Chromaster Ultra Rs-Fornitore globale

Room 801, Building 2, Dexin Digital Industry Building, No. 2008 and 2010 Jinchang Road, Liangzhu Street, Yuhang District, Hangzhou City, Zhejiang Province

Product Introduction

Hitachi Ultra High Performance Liquid Chromatography System Chromaster Ultra Rs

Hitachi Ultra High Performance Liquid Chromatography (UHPLC) can fully meet the requirements of cutting-edge analysis

As is well known, the research and development departments in the fields of medicine and chemistry have very high requirements for instrument performance, resolution, and sensitivity, and the Chromaster Ultra Rs ultra-high performance liquid chromatograph can fully meet the requirements of these users. Typical cutting-edge analyses nowadays, such as high-resolution analysis of impurities in chemically similar and synthetic compounds, and high-sensitivity detection of harmful substances and other impurities closely related to daily life, all place high demands on instrument performance, and Hitachi Ultra High Performance Liquid Chromatography (UHPLC) can fully meet these requirements.

characteristic

	Ultra high speed analysis
	Ultra high pressure resistance level: 140 MPa, which can truly achieve ultra high speed analysis. Due to the increased pressure resistance of the system, some mobile phases that can cause pressure rise can also be used, which expands the range of optional analysis methods and is very suitable for the development of detection and analysis methods for complex components.

	High-resolution analysis
	The newly developed chromatographic column (LaChromUltra II ODS C18, with a filler particle size of 1.9 μ m); m， With a length of 250 mm, the theoretical number of trays is over 50000, and the pressure resistance is up to 140 MPa, achieving true ultra-high resolution analysis. The new binary pump is equipped with a variable stroke independent plunger, in addition to Hitachi's unique LBT^1Technology and standard low capacity double threaded mixer ensure better mixing effect and stability during liquid delivery. A 10mm total reflection capillary flow cell can effectively reduce out of column diffusion and achieve higher resolution.*

	High sensitivity analysis
	The 65mm flow cell of the diode array detector (optional) has extremely high sensitivity and is particularly outstanding for detecting trace substances. The new optical system of the diode array (DAD) detector effectively reduces noise and drift, ensuring high sensitivity analysis results. The automatic sampler is equipped with multiple cleaning modes and comes with a standard injection port backwash function, greatly reducing sample residue.

Module product line

Diode Array (DAD) Detector System
UV-VIS detector system

Application examples

	Peptide mapping method for hydrolyzed protein


	New chromatographic column (250 mm long, packing particle size 1.9 μ m); m) The theoretical number of trays and peak capacity are higher. This chromatographic column can withstand higher pressure and has a packing particle size of 5 μ m; Compared to the chromatography column with the same length, the analysis time is reduced by half and the peak capacity is increased by 1.4 times.

	Chromatographic conditions
	Mobile phase (A) 0.1% - TFA/H2O (B) 0.1% - TFA/CH3CN gradient (1) 5% B (0 min)→ 45％ B（60 min）（2）5％ B（0 min）→ 45% B (30 min) flow rate (1) 1.0 mL/min (2) 0.85 mL/min column temperature 40 ° C; C detection wavelength 215 nm injection volume (1) 10µ L (about 100µ g）（2）5 µ L (approximately 50 μ m); g）

*1：

LBT： Liquid compression technology