Hitachi UHPLC chromatography column LaChromUltraII C18
It combines super durability, ultra-high pressure resistance, and high separation performance.
The application scope of UHPLC has expanded from ultra-high speed analysis to ultra-high separation analysis.
LaChromUltra II adopts inorganic organic composite silicon materials with higher physical and chemical resistance than the original silicone particles. Hitachi High Tech has launched a chromatography column with a particle size of 1.9 µ m and a length of 250mm, which maximizes the pressure resistance of UHPLC.
Therefore, the application scope of UHPLC has also expanded from ultra high speed analysis to high separation analysis.
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characteristic
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technical parameter
characteristic
The inorganic organic composite silicon material with higher physical and chemical resistance than the original silicone particles has excellent pressure resistance. Users can choose a 250mm chromatographic column. The theoretical number of trays can reach over 50000, which can play an advantage in the analysis of related substances and multi-component analysis.
Moreover, if two 250mm chromatography columns are used, the theoretical number of plates can reach a ultra-high separation of 100000.
LaChromUltra II C18 (1.9 µ m) Measurement Example

Analysis conditions:
| mobile phase | (1) (2) 60% CH3CN (3) (4) 70% CH3CN |
|---|---|
| Column temperature | 40℃ |
| Detection wavelength | UV 270 nm |
| Injection volume | (1)(2)1 µL (3)(4)2 µL |
| instrument |
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Sample:
- Uracil
- Methyl benzoate
- naphthalene
- Butyl benzoate
technical parameter
| name | Particle size (µ m) | Column size (mm I.D. × mm L.) |
|---|---|---|
|
LaChromUltra II C18(1.9 µm) The properties of the filler are the same as LaChromium II C18. Easy conversion between ultra high speed LC and conventional LC methods. |
1.9 | 2 ×50 |
| 2 ×100 | ||
| 2 ×150 | ||
| 3 ×50 | ||
| 3 ×100 | ||
| 3 ×150 | ||
| 3 ×250 | ||
| 4.6 ×250 |
| name | Particle size (µ m) | Column size (mm I.D. × mm L.) |
|---|---|---|
|
LaChrom II C18 The filler properties are the same as LaChromUltra II C18. Easy conversion between ultra high speed LC and conventional LC methods. |
3 | 4.6 ×100 |
| 4.6 ×150 | ||
| 5 | 4.6 ×150 | |
| 4.6 ×250 |

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