FluorCamPortable GFP/Chl. fluorescence imaging instrument

FluorCamThe portable GFP/Chl. fluorescence imaging instrument is an extended version of the FluorCam portable chlorophyll fluorescence imaging instrument, which can be used not only for chlorophyll fluorescence imaging, but also for imaging and analysis of the heterogeneity of green fluorescent protein (GFP) distribution in plants, animals or their tissues, organs and colonies. Four sets of high brightness light-emitting diodes provide high-intensity measurement light or moderate sustainable light, and high-intensity measurement light pulses can enable fluorescence signal imaging measurement to be carried out under high background light. The fluorescence signal is detected and imaged by a high-sensitivity CCD camera, and the dynamic fluorescence image can be analyzed by the accompanying software. The measurement samples can be small plants, small animals, green algae, bacterial colonies, and other living biological fluorescence imaging.

Functional features:

§ Strong portability, suitable for both laboratory and field use

§ Imageable measurement of GFP (green fluorescent protein) and chlorophyll fluorescence dynamics

§ You can edit the measurement experiment protocol by yourself

§ It can perform both continuous photochemical imaging measurement and PAM imaging measurement

§ Equipped with dark adaptation leaf clips, no damage to the sample

§ High resolution lens, snapshot mode, video mode (with binning mode)

§ Optional handheld chlorophyll rapid fluorescence kinetics measurement module

Typical sample

• Algae such as kelp, horsetail algae, seaweed, blue-green algae communities, etc

· Lichens, mosses, crusts, etc

· Whole small plants, such as Arabidopsis, etc

· Plant canopy, leaves or fruits, such as strawberries, cucumbers, tomatoes, lemons, melons, etc

· Other photosynthetic organisms, small animals expressing GFP

Technical parameters:

• Measurement parameters:Fo, Fo’, Fs, Fm, Fm’, Fp, FtDn, FtLn, Fv, Fv'/ Fm'， Fv/ Fm ,Fv',Ft,ΦPSII,NPQ_Dn, NPQ_Ln, Qp_Dn, Qp_Ln, qN, qP，QY, QY_Ln,Rfd, ETR Waiting for over 50 chlorophyll fluorescence parameters, each parameter can display a 2D fluorescence color image

· Equipped with a complete automatic measurement program (protocol), the automatic measurement program can be freely edited

a) Fv/FmMeasurement parameters include Fo, Fm, Fv, QY, etc

b) KautskyInduction effect: Fluorescence parameters such as Fo, Fp, Fv, Ft_Lss, QY, Rfd, etc

c) Fluorescence quenching analysis: Fo, Fm, Fp, Fs, Fv, QY,Φ_II， More than 50 parameters including NPQ, Qp, Rfd, qL, etc

d) Light response curve LC: Fluorescence parameters such as Fo, Fm, QY, QYLn, ETR, etc

e) GFPStatic fluorescence imaging measurement

§ High resolution TOMI-2 CCD sensor

a) Line by line scanning CCD

b) Maximum image resolution: 1360 × 1024 pixels

c) Time resolution: up to 20 frames per second at the highest image resolution

d) A/DConversion resolution: 16 bits (65536 grayscale levels)

e) Pixel size: 6.45 µ m × 6.45 µ m

f) Operation mode: 1) Dynamic video mode, used for measuring chlorophyll fluorescence parameters; 2) Snapshot mode, used for measuring fluorescent proteins such as GFP and fluorescent dyes

g) Communication mode: Gigabit Ethernet

§ CCDFilter: Red chlorophyll fluorescence specific filter, green GFP specific filter

§ Imaging area: 35mm × 46mm

§ Light source board: 4 ultra bright LED light source boards, each light source board consists of a 5 × 5 LED array, with a size of 4 × 4 cm

§ Measurement light: 620nm red light, duration adjustable from 10 µ s to 100 µ s

§ Saturated light: 455nm blue light, maximum intensity 4900 µ mol (photons)/m ². s

§ Photochemical light: 455nm blue light, maximum intensity of 1400 µ mol (photons)/m ². s

§ Far red light: 735nm, used for measurementFo’, 4 high-energy LEDs

§ OJIP–test(Optional): It can analyze the rapid fluorescence dynamic photochemical and thermal phases of plants

§ FluorCamChlorophyll fluorescence imaging analysis software features: Live (real-time testing), Protocols (customized experimental program selection), Pre processing (imaging preprocessing), Result (imaging analysis results) and other functional menus

§ Customized experimental program protocols for customers: can set time (such as measurement light duration, photochemical light duration, measurement time, etc.), light intensity (such as photochemical light intensity of different qualities, saturation light flash intensity, modulated measurement light, etc.), with dedicated experimental program language and scripts. Users can also freely create new experimental programs using the wizard program template in the Protocol menu

§ Automatic measurement and analysis function: An experimental program (Protocol) can be set to automatically perform unmanned cyclic imaging measurement,The number of repetitions and interval time can be customized by the customer,The imaging measurement data is automatically stored in the computer according to the time and date (with timestamp)

§ Snapshot mode: Through snapshot imaging mode,Clear and prominent steady-state and instantaneous fluorescence images of plant samples can be obtained by freely adjusting the light intensity, shutter time, and sensitivity

§ Imaging preprocessing: The program software can automatically identify multiple plant samples or regions, or manually select regions of interest (ROI). The shape of manual selection can be square, circular, any polygon, or sector. The software can automatically measure and analyze the fluorescence kinetics curves and corresponding parameters of each sample and selected area, with no limit on the number of samples or areas (>1000)

§ Data analysis mode: It has "signal calculation and re averaging" mode (arithmetic mean) and "signal average and re calculation" mode. In high signal-to-noise ratio situations, the "signal calculation and re averaging" mode is selected, and in low signal-to-noise ratio situations, the "signal average and re calculation" mode is selected to filter out errors caused by noise

§ Output results: High time resolution fluorescence dynamic image, fluorescence dynamic change video, fluorescence parameter Excel file, histogram, imaging images with different parameters, fluorescence parameter list for different ROIs, etc

§ Give Light System:Static or dynamic (sinus style)

§ CCDDetection range:400–1000nm

§ Spectral response:540nmThe quantum efficiency is highest at 70%, with a 50% decrease at 400nm and 650nm

§ Read out the noise:Below 12eRMS, typical 10e

§ Full trap capacity:Greater than 70000 e (unbound)

§ Bios：Firmware upgradable

§ Communication method:Gigabit Ethernet

§ Host weight:1.8 kg

§ Host size:21.5 cm×13.5 cm×13.5 cm

§ Leaf clamp: used to clamp measuring blades and perform dark adaptation

§ Bracket system: 1) Indoor bracket, adjustable measurement height and angle, used for laboratory measurements; 2) Triangle bracket (optional), designed with waterproof and rust proof materials to meet measurement stability, adjustable height angle, maximum measurement height of 1.5m, used for field measurement

§ Power supply method: 1) 90-240 V AC power, equipped with a dedicated surge resistant and stabilized power supply; 2) Specialized field battery pack (optional), capable of supporting uninterrupted measurement for over 10 hours on a single charge

§ Maximum power: 200 W

Place of Origin:Europe