FluorCamMobile Plant Multispectral Fluorescence Imaging System

The FluorCam mobile multispectral fluorescence imaging system is an advanced extension product of the internationally renowned FluorCam chlorophyll fluorescence imaging technology. It can be used for dynamic imaging analysis of chlorophyll fluorescence, multi excitation light photosynthetic efficiency imaging analysis, UV excited multispectral fluorescence imaging analysis, PAR absorption and NDVI (Plant Spectral Reflectance Index) imaging analysis, GFP/YFP steady-state fluorescence imaging, etc. It comprehensively, non-contact, and high-sensitivity reflects plant physiological ecology, stress physiology and resistance, photosynthetic efficiency, etc. It is widely used in plant phenotype imaging analysis, plant stress and resistance detection, plant disease and case detection research, high-throughput screening in genetic breeding, plant physiological ecology, plant primary metabolism and stimulus metabolism research, pollution ecology research detection/biological detection, etc.

Main functional features:

ü Customized four-wheel mobile platform and modular structure for easy use in greenhouses or outdoors, in-situ measurement and monitoring, system expansion, etc

ü Chlorophyll fluorescence imaging analysis:Measurement and detection of physiological functions such as plant photosynthetic efficiency, fluorescence quenching heat dissipation, light response curve, plant stress and resistance

ü Multispectral fluorescence imaging analysis:Reflect the dynamic changes of secondary metabolites such as polyphenols and flavonoids, chlorophyll, plant aging, plant pest and disease stress, and abiotic stress

ü GFP/YFPSteady state fluorescence imaging (optional): genetic breeding, gene labeling

ü NDVIImaging analysis (optional):NNutritional status, plant stress, etc

üUV induced multispectral fluorescence imaging technology:UV light can excite plant leaves and produce a fluorescence spectrum with four characteristic peaks. The wavelengths of the four peaks are blue light at 440nm (F440), green light at 520nm (F520), red light at 690nm (F690), and far-infrared light at 740nm (F740). F440 and F520 are collectively referred to as BGF, which is emitted by the epidermis, mesophyll cell walls, and leaf veins. F690 and F740 are chlorophyll fluorescence Chl-F. UV induced multispectral fluorescence can be used to sensitively and specifically evaluate plant physiological states, including stress states such as drought, pests and diseases, environmental pollution, nitrogen stress, etc

ü Multi excitation luminescence and multispectral fluorescence imaging technologyThrough optical filter technology, only specific wavelengths of light (excitation light) are allowed to reach the sample to excite fluorescence, while only specific wavelengths of excitation fluorescence are allowed to reach the detector. Different fluorescent chromophores (such as chlorophyll or...)GFPGreen fluorescent protein (GFP) is "sensitive" to excitation light of different wavelengths and absorbs it to excite fluorescence of different wavelengths. Based on this principle, it can be optionally selected2A or2Multiple excitation light sources, green wave wheels, and corresponding filters are used for imaging analysis of fluorescence at different wavelengths (multispectral fluorescence). If red and blue light and corresponding filters are selected, it can be used toGFPIn addition to chlorophyll fluorescence imaging analysis, green light sources and corresponding filters can also be selected for analysisYFPPerform fluorescence imaging analysis, etc;

ü Optional13x13cmOr large20x20cmDifferent specifications and modelsLEDLight source version, to achieve uniform plant multispectral fluorescence imaging areas of different sizes, can be used for experimental imaging analysis of leaves, plant fruit organs, including plant roots, whole plants, and even multiple plants

ü Can perform automatic repeated imaging measurement and unmanned monitoring, and can set up two experimental programs（Protocols）Automatic loop imaging measurement, imaging measurement data is automatically stored in the computer by time and date (with timestamp) for long-term (such as several hours) automatic monitoring in the field

ü haveKautskyInduction effect, fluorescence quenching analysisGFPVarious general experimental procedures such as steady-state fluorescence imaging and UV excited multispectral fluorescence imaging analysis（protocols）Measuring and analyzing parameters60Multiple

ü OptionalRGBImaging, infrared thermal imaging, and hyperspectral imaging are used for fusion analysis with chlorophyll fluorescence imaging to comprehensively study and analyze morphological structure, color, stomatal conductance, plant spectral characteristicsWUE(Water use efficiency)CWSI(Water stress index), plant physiology and biochemistry, etc

ü Measurement samples include leaves, flowers, fruits, roots, other plant tissues, whole plants, algae, small animals, etc

Technical indicators:

1. Multispectral fluorescence imaging

1)Standard configuration4+1intelligenceLEDLight source system, including1Regarding the color redLEDLight source board1For cold whiteLEDLight source board1Top UV light source, optional with other wavelength light sources and multispectral light sources

2) Standard versionLEDLight source board area13x13cm, Large EditionLEDThe area of the light source board reaches20x20cm

3)pulseModulation measurement light source, wavelength 617nm, duration adjustable from 10 µ s to 100 µ s, can penetrate deep into leaf fence tissue, optional with blue or other color measurement light

4) Dual band continuous photochemical light: standard 617nm orange photochemical light and 6500K white photochemical light, with adjustable light intensity of 300-2000 µ mol (photons)/m ². s, optional light intensity of 3000 µ mol (photons)/m ². s, and other colors of photochemical light such as blue and green can also be selected

5) 6500K white saturated light pulse, maximum intensity 4000 µ mol (photons)/m ². s, optional intensity up to 6000 µ mol (photons)/m ². s, blue saturated light pulse or 120000 µ mol (photons)/m ². s single cycle light pulse can also be selected

6) Protocols: Includes (depending on optional configuration):

a)Fv/Fm

b) Kautsky induced effect

c) Fluorescence quenching analysis

d) Analysis of Light Response Curve

e) Static fluorescence imaging analysis (optional)

f) Multispectral Fluorescence Imaging Analysis

g) PARabs/NDVI imaging analysis (optional)

7) The measurement parameters include Fo, Fo’, Fs, Fm, Fm’, Fp, FtDn, FtLn, Fv, NPQ_Dn, NPQ_Ln, Qp_Dn, Qp_Ln, qN, QY, QY_Ln, PARabs, Rfd，BGF，UV-Chl.F Waiting for over 60 chlorophyll fluorescence parameters and multispectral fluorescence parameters

8) UV excited multispectral fluorescence parameters include F440, F520, F690, F740, and corresponding ratio imaging analysis parameters (the left figure below shows RGB color imaging and multispectral fluorescence imaging of soft rot pathogen (Dickeya dadantii) infected zucchini (quoted from Maria L. Perez Bueno et al., 2016); The right image shows the UV induced multispectral fluorescence imaging of plants inoculated with viruses (PMMoV-I is an Italian strain, PMMoV-S is a Spanish strain) (dpi is the number of infection days after inoculation), where Abaxia is the imaging of the back of the leaf and Adaxia is the imaging of the front of the leaf (quoted from Monica Pineda et al., 2008)

9) High resolution CCD lens

a) Maximum image resolution: 1360 × 1024 pixels

b) Time resolution: up to 20 frames per second at the highest image resolution

c) A/D conversion resolution: 16 bits (65536 grayscale levels)

d) Pixel size: 6.45 µ m × 6.45 µ m

10) 7-bit filtering wheel and filter, used for imaging measurement of chlorophyll fluorescence, F440, F520, F690, F740, and steady-state fluorescence such as GFP (GFP fluorescence requires corresponding functional modules to be selected)

11) Optional dual color LED light source boards with 740nm far red light (FAR) and 660nm, corresponding filters, and functional program modules for measuring Fo ', PARabs, and NDVI

12) Optional configuration includes 1 pair of blue LED light source boards and corresponding filters, which can be used for measuring and researching pore function

13) Optional 1 pair of green LED light source boards for measuring YFG (corresponding filters must be selected)

14) If measuring other fluorescence parameters, corresponding filters must be selected (please consult EcoLab laboratory). The following are selection references:

15) FluorCam chlorophyll fluorescence imaging analysis software features: Live (live testing), Protocols (customized experimental program selection), Pre processing (imaging preprocessing), Result (imaging analysis results) and other menu functions

16) Customized experimental program protocols for customers: can set time (such as measurement light duration, photochemical light duration, measurement time, etc.), light intensity (such as photochemical light intensity of different qualities, saturation light flash intensity, modulated measurement light, etc.), with dedicated experimental program language and scripts. Users can also freely create new experimental programs using the wizard program template in the Protocol menu

17) Automatic measurement and analysis function: An experimental program (Protocol) can be set to automatically perform unmanned cyclic imaging measurements. The number of repetitions and interval time can be customized by the customer, and the imaging measurement data will be automatically stored in the computer according to the time and date (with a timestamp)

18) Snapshot mode: Through snapshot imaging mode, the light intensity, shutter time, and sensitivity can be freely adjusted to obtain clear and prominent steady-state and instantaneous fluorescence images of plant samples

19) Imaging preprocessing: The program software can automatically identify multiple plant samples or regions, or manually select regions of interest (ROI). The shape of manual selection can be square, circular, any polygon, or sector. The software can automatically measure and analyze the fluorescence kinetics curves and corresponding parameters of each sample and selected area, with no limit on the number of samples or areas (>1000)

20) Data analysis mode: It has "signal calculation and re averaging" mode (arithmetic mean) and "signal average and re calculation" mode. In high signal-to-noise ratio situations, the "signal calculation and re averaging" mode is selected, and in low signal-to-noise ratio situations, the "signal average and re calculation" mode is selected to filter out errors caused by noise

21) Lighting system: static or dynamic

22) Bios: Firmware upgradable

23) Communication method: Gigabit Ethernet

24) Power supply voltage: 90-240 V (a portable generator is required for outdoor use)

2. Infrared thermal imaging unit:

1) Uncooled VOx microbolometer, calibrated according to EU standards, can directly measure temperature, including the temperature of each pixel

2) Resolution: 640x512 pixels

3) Spectral range: 7.5-13.5 μ m

4) Temperature measurement range:- 25～150°C


5) Sensitivity: ≤ 0.03℃（30mK）@ 30℃

6) Frame rate: Standard configuration of 9Hz or 30Hz (optional must be applied for in advance)

7) Data transmission: USB-3 or Gigabit Ethernet

8) 19mm optical lens, field of view 32 ℃ x26 ℃, optional 13mm or 35mm lens

9) Equipped with video mode and snapshot mode

10) Equipped with 14 color palettes for arbitrary selection, allowing for diverse settings of thermal imaging false colors

11) Equipped with differential function, it can internally check the image to form a smooth image to avoid pixelation

12) Parameters such as atmospheric temperature, humidity, and distance can be set through software

13) Equipped with isothermal mode function, including the above, below, between, and below modes, as well as the above four isothermal modes

14) The online reporting function automatically displays thermal images, time distance maps, and image parameters such as emissivity, reflection temperature, atmospheric temperature, humidity, external light distance, propagation, etc

15) The image processing software has ROI selection function, including points, lines, polylines, rectangles, etc., and can perform partition processing. Each ROI displays the minimum temperature, maximum temperature, average temperature, etc. in real time

16) Thermal scanning function and thermal profile function: online visualization display of linear ROI temperature values and temperature profile maps

17) The temperature values of all ROI tools can be displayed in the time distance graph

18) Protection level: IP65

19) Working temperature:-15°C～+50°C

20) Supports GPS information and can display location information on Google Maps

3. RGBImaging analysis unit:Research grade RGB imaging lens, resolution 2592x1944 pixels, signal-to-noise ratio 54dB, 1-40x magnification, minimum field of view 6.1x7.9mm (40x), maximum field of view 20.8x25.4

Place of Origin:Europe