FluorCam Large Chlorophyll Fluorescence Imaging Platform

FluorCamThe large-scale chlorophyll fluorescence imaging platform is the world's largest single image chlorophyll fluorescence imaging system, with a field mobile platform (see "Fluorcam Field Mobile Large Chlorophyll Fluorescence Imaging SystemThere are two versions, "Tong" and "Laboratory Platform" (this introduction), which integrate LED excitation light source, CCD chlorophyll fluorescence imaging lens, and filtering wheel into a highly movable imaging platform. It is suitable for laboratory or greenhouse plant physiological and ecological research, plant phenotype imaging analysis (Phenotyping), and plant stress analysisPhysiological and resistance screening, plant genetic breeding research, seed germination and vitality monitoring, plant ecotoxicology research, can perform high-throughput imaging analysis on the entire plant or plant community. （In the figure on the right, 1 is the FluorCam imaging platform, 2 is the PAR absorption/NDVI module, 3 is the CCD detector, 4 is the main power switch, 5 is the main power supply, 6 is the imaging platform power line, 7 is the main power line, 8 is the data line, and 9 is the system bracket）

Functional features:

1) It is the chlorophyll fluorescence imaging system with the largest single imaging area in the world, with an imaging area of35×35cm,Non damaging chlorophyll fluorescence imaging analysis can be performed on the entire plant and multiple plants simultaneously

2) The imaging platform is adjustable in height to adapt to plant imaging analysis at different heights

3) Optional PAR absorption/NDVI imaging analysis module for imaging analysis of plant PAR absorption and spectral reflectance index NDVI

4) OptionalRGBImaging analysis module, used for plant morphology measurement and analysis, etc

5) Optional GFP green fluorescent protein imaging analysis function for plant transgenic research detection

6) Can perform automatic repeated imaging measurements through experimental procedures（Protocols)Set the number of repeated measurements and time intervals to achieve automatic cyclic imaging measurement. The imaging measurement data is automatically stored in the computer according to the time and date (with a timestamp), thus realizing unmanned automatic monitoring function

Technical indicators:

· Measurement parameters: Fo, Fo’, Fs, Fm, Fm’, Fp, FtDn, FtLn, Fv, Fv'/ Fm'，Fv/ Fm ,Fv',Ft,ΦPSII, NPQ_Dn, NPQ_Ln, Qp_Dn, Qp_Ln, qN, qP，QY, QY_Ln, Rfd, ETR Waiting for over 50 chlorophyll fluorescence parameters, PAR absorption, and NDVI plant spectral reflectance indices, each parameter can display a 2D fluorescence color image

· Equipped with a complete automatic measurement program (protocol), the automatic measurement program can be freely edited

a) Fv/FmMeasurement parameters include Fo, Fm, Fv, QY, etc

b) KautskyInduction effect: Fluorescence parameters such as Fo, Fp, Fv, Ft_Lss, QY, Rfd, etc

c) Fluorescence quenching analysis: Fo，Fm，Fp，Fs，Fv，QY，Φ_II， More than 50 parameters including NPQ, Qp, Rfd, qL, etc

d) Light response curve LC: Fluorescence parameters such as Fo, Fm, QY, QYLn, ETR, etc

e) PARAbsorption and NDVI (optional)

f) GFPStatic fluorescence imaging measurement (optional)

• high sensitivityTOMI-1CCDSensor:

a) Image resolution: 720 × 560 pixels

b) Time resolution: 50 frames per second

c) A/DConversion resolution: 12 bits (4096 grayscale levels)

d) Pixel size: 8.6 µ m × 8.3 µ m

e) Communication mode: Gigabit Ethernet

§ High resolution TOMI-2 CCD sensor (optional)

a) Line by line scanning CCD

b) Maximum image resolution: 1360 × 1024 pixels

c) Time resolution: up to 20 frames per second at the highest image resolution

d) A/DConversion resolution: 16 bits (65536 grayscale levels)

e) Pixel size: 6.45 µ m × 6.45 µ m

f) Operation mode: 1) Dynamic video mode, used for measuring chlorophyll fluorescence parameters; 2) Snapshot mode, used for measuring fluorescent proteins such as GFP and fluorescent dyes

g) Communication mode: Gigabit Ethernet

§ Light source board: standard 620nm red measurement light source, 620nm and cool white dual color chemical light source (optional blue or other wavelength LED light source), equipped with 735nm infrared light source,LEDLight source board area 730×730mm

§ Adjustable range of imaging platform height: 0-1500mm, imaging distance (distance from the platform to the top of the plant) 220-250mm

§ Bichromatic light: Actinic1 has a maximum light intensity of 300 µ mol (photons)/m ². s, while Actinic2 has a maximum light intensity of 500 µ mol (photons)/m ². s; The maximum photochemical intensity can be upgraded to Actinic1 500 µ mol (photons)/m ². s and Actinic2 1500 µ mol (photons)/m ². s

§ Saturated flicker: maximum light intensity of 3000 µ mol (photons)/m ². s, upgradable to 6000 µ mol (photons)/m ². s

§ Imaging area: 35 × 35cm, capable of imaging analysis of 96 well plates, 384 well plates, plant leaves, plant tissues, algae, mosses, lichens, whole plants, multiple plants, and even plant irrigation layers

§ GFPMeasurement module (optional): 7-bit filtering wheel and dedicated filter for measuring GFP (green fluorescent protein)

§ PARAbsorption measurement module (optional): Far red light 740nm (FAR), 660nm dual color LED light source board and dedicated filter for measuring PAR absorption and NDVI

§ FluorCamChlorophyll fluorescence imaging analysis software features: Live (real-time testing), Protocols (customized experimental program selection), Pre processing (imaging preprocessing), Result (imaging analysis results) and other functional menus

§ Customized experimental program protocols for customers: can set time (such as measurement light duration, photochemical light duration, measurement time, etc.), light intensity (such as photochemical light intensity of different qualities, saturation light flash intensity, modulated measurement light, etc.), with dedicated experimental program language and scripts. Users can also freely create new experimental programs using the wizard program template in the Protocol menu

§ Automatic measurement and analysis function: An experimental program (Protocol) can be set to automatically perform unmanned cyclic imaging measurement,The number of repetitions and interval time can be customized by the customer,The imaging measurement data is automatically stored in the computer according to the time and date (with timestamp)

§ Snapshot mode: Through snapshot imaging mode,Clear and prominent steady-state and instantaneous fluorescence images of plant samples can be obtained by freely adjusting the light intensity, shutter time, and sensitivity

§ Imaging preprocessing: The program software can automatically identify multiple plant samples or regions, or manually select regions of interest (ROI). The shape of manual selection can be square, circular, any polygon, or sector. The software can automatically measure and analyze the fluorescence kinetics curves and corresponding parameters of each sample and selected area, with no limit on the number of samples or areas (>1000)

§ Data analysis mode: It has "signal calculation and re averaging" mode (arithmetic mean) and "signal average and re calculation" mode. In high signal-to-noise ratio situations, the "signal calculation and re averaging" mode is selected, and in low signal-to-noise ratio situations, the "signal average and re calculation" mode is selected to filter out errors caused by noise

§ Output results: High time resolution fluorescence dynamic image, fluorescence dynamic change video, fluorescence parameter Excel file, histogram, imaging images with different parameters, fluorescence parameter list for different ROIs, etc

Customized model:

Standard open version closed version (can be equipped with GMS 150 gas mixing system to regulate CO)₂Concentration)

XYZ mobile scanning version (can be equipped with thermal imaging and RGB color imaging) Step in cultivation room version (can be equipped with thermal imaging and RGB color imaging)

Origin: Europe