FP-leafLeaf clip plant spectrum and chlorophyll fluorescence measurement package

FP-leafThe leaf clip type plant spectrum and chlorophyll fluorescence measurement kit is used to measure plant chlorophyll fluorescence, leaf reflectance spectra, and spectral indices at the leaf level, including handheld chlorophyll fluorescence measuring instruments and plant reflectance spectroscopy measuring instruments. Suitable for rapid detection of a large number of samples in the wild, widely used in plant stress response, herbicide detection, ecological toxicology biological detection, plant reflectance spectroscopy measurement, pigment composition changes, nitrogen content changes, yield estimation, ecology, molecular biology, etc.

The measured data is displayed in real-time on the instrument's display screen in the form of graphics or data tables. These data can be stored in the instrument's memory and transmitted to the computer. The measuring instrument is powered by a rechargeable lithium battery,It can be measured independently without the need for a computer. The measuring instrument is equipped with a full-color touchscreen display, built-in light source, built-in GPS, and non-destructive blade clamp for fixing samples.

application area

Suitable for photosynthesis research and teaching, plant and molecular biology research, agriculture, forestry, biotechnology fields, etc. The research content involves photosynthetic activity, stress response, pesticide efficacy testing, mutation screening, pigment content evaluation, etc.

·Research on Plant Photosynthetic Characteristics

·Screening of photosynthetic mutants and phenotype research

·Detection of biotic and abiotic stress

·Research on Plant Stress Resistance or Susceptibility

·Agricultural and forestry breeding, disease detection, growth and yield evaluation

·Herbicide testing

·Changes in pigment composition

·Changes in nitrogen content

·Production estimation

·teaching

Functional Features

§Compact in structure and highly portable, the light source, detector, and control unit are integrated into a smartphone sized instrument

§Powerful functionality, equipped with all the functions of a large-scale chlorophyll fluorescence analyzer and reflectance spectrometer, capable of measuring all chlorophyll fluorescence parameters and automatically calculating commonly used plant reflectance spectral indices, while providing fluorescence kinetics curves and high-precision reflectance spectra

§Chlorophyll fluorescence detection is equipped with all common experimental programs, including 3 sets of fluorescence quenching analysis programs, 3 sets of light response curve programs, OJIP rapid fluorescence kinetics curve, etc

§Chlorophyll fluorescence detection has high time resolution, up to 100000 times per second, automatically draws OJIP curves and provides 26 OJIP test parameters

§Professional software with powerful functions: chlorophyll fluorescence analysis software can download and display chlorophyll fluorescence parameter charts, and can also directly control instruments for measurement through the software; Plant spectral analysis software can automatically calculate built-in vegetation indices, calculate user-defined vegetation indices, and display real-time data graphs and tables

§Chlorophyll fluorescence detection has unmanned automatic monitoring function

§Equipped with GPS module, outputting chlorophyll fluorescence parameter charts and reflectance spectrum data with timestamps and geographic locations

technical parameter

1. Measurement parameters and procedures

1.1Chlorophyll fluorescence measurement includes more than 50 chlorophyll fluorescence parameters such as F0, Ft, Fm, Fm ', QY, QY1, Ln, QY1, Dn, NPQ, Qp, Rfd, PAR (limited to PAR models), Area, Mo, Sm, PI, ABS/RC, etc

1.2Chlorophyll fluorescence OJIP test includes F0 Fj、Fi、Fm、Fv、Vj、Vi、Fm/F0、Fv/F0、Fv/Fm、Mo、Area、Fix Area、Sm、Ss、N、Phi_Po、Psi_o、Phi_Eo、Phi–Do、Phi_Pav、PI_Abs、ABS/RC、TRo/RC、ETo/RC、DIo/RC etc.

1.3Chlorophyll fluorescence measurement program: Ft, QY, OJIP, NPQ1, NPQ2, NPQ3, LC1, LC2, LC3, PAR (limited to PAR models), Multi unmanned automatic monitoring

1.4Vegetation Reflectance Index: NDVI、SR、 Greenness index, MCARI, TCARI, TVI, ZMI, SRPI, NPQI, PRI, NPCI, Carter index, SIPI, GM1, SR, MCARI1, OSAVI, MCARI, Ctr2, GM2 (depending on the specific model)

2. Handheld chlorophyll fluorescence measurement unit:

2.1Type of blade clamp: fixed blade clamp, separated blade clamp, probe type, etc

2.2PARSensor: cosine correction for 80 º incident angle, reading unit µ mol (photons)/m ². s, can display readings, detection range 400-700 nm

2.3 Measurement light: up to 0.09 µ mol (photons)/m ². s per measurement pulse, adjustable from 10-100%

2.4Photochemical light: 10-1000 µ mol (photons)/m ². s adjustable

2.5Saturated light: up to 3000 µ mol (photons)/m ². s, adjustable from 11-100%

2.6Light source: Standard configuration blue light 455nm, LED light sources with different wavelengths can be equipped according to needs

2.7Size: Ultra portable, phone size, 134 × 65 × 33mm (excluding probe), weight only 188g

2.8Data storage: Capacity of 16Mb, capable of storing 149000 data points

2.9Display and operation: graphical display, double key operation, automatic shutdown after 5 minutes of standby

2.10Power supply: 2000mA rechargeable lithium battery, USB charging, can work continuously for 48 hours, low battery alarm

2.11Working conditions: 0-55 ℃, 0-95% relative humidity (without condensation)

2.12Storage conditions: -10-60 ℃, 0-95% relative humidity (without condensation)

2.13Communication method: Bluetooth+USB dual communication mode, Bluetooth has a maximum transmission speed of 3Mbps at a distance of 20m

2.14GPSModule: Built in, with a maximum accuracy of 1.5m

2.15Software: FluorPen 1.1 dedicated software, used for data download, analysis, and chart display, outputting Excel data files and fluorescence kinetics curve graphs

3. Handheld plant reflectance spectroscopy unit

3.1Spectral detection range:

PolyPen RP 410 UVISThe spectral response range is 380-790nm

PolyPen RP 410 NIRThe spectral response range is 640-1050nm

3.2Light source: Xenon incandescent lamp 380-1050nm

3.3Spectral response half width: 8nm

3.4Spectral stray light:- 30dB

3.5Optical aperture: 7mm

3.6Scanning speed: approximately 100ms

3.7Touch screen: 240 × 320 pixels, 65535 colors

3.8Memory: 16MB (capable of storing over 4000 sets of measurement data)

3.9System data: 16 bit analog-to-digital conversion

3.10Dynamic range: high gain 1:4300; Low gain 1:13000

3.11Built in GPS module: maximum accuracy<1.5m

3.12Communication method: USB

3.13Software features: Automatic calculation of built-in vegetation index, calculation of user-defined vegetation index, real-time display of data graphs and tables, data export to Excel, GPS map, firmware upgrade, suitable for Windows XP and above systems

3.14Spectral reflectance standard accessory (optional): Provides the highest diffuse reflectance value (99%). The spectral plane covers UV-VIS-NIR spectra, ensuring an optical plane of+/-1%. Used for calibration of light sources and detectors.

3.15Size: 15 × 7.5 × 4cm

3.16Weight: 300g

3.17Shell: Waterproof splash shell

3.18Battery: 2600mAh rechargeable lithium battery, connected to a computer via USB interface for charging

3.19Battery life: can measure continuously for 48 hours

3.20Working conditions: Temperature range of 0-55 ℃, relative humidity range of 0-95% (without condensation)

3.21Storage conditions: Temperature -10~60 ℃, relative humidity 0-95% (no condensation water)

Application Case 1:

The Joint Research Centre of the European Commission is using drone telemetry technology to study the infection of leaf blight pathogen in olive trees. At the same time, the chlorophyll fluorescence and reflectance spectrum vegetation index of the leaves were directly detected by FluorPen chlorophyll fluorescence analyzer and RP400 spectrometer, which were used to compare and correct the drone telemetry data. The research results were published in Nature Plants (Zarco Tejada, 2018).

Application Case 2:

The high temperature at night during the grain filling period of rice can significantly affect the yield of rice. The Global Change Research Center of the Czech Academy of Sciences collaborated with the International Rice Research Institute to study the tracking of changes in optical properties of mature rice panicles under high nighttime temperatures. The researchers used a FluorPen handheld chlorophyll fluorescence meter to measure the effective photochemical efficiency Φ II (also known as effective quantum yield QY or Φ PSII) and steady-state fluorescence Fs of the photosynthetic system. At the same time, the previous model WinePen of PolyPen handheld plant reflectance spectrometer was used to measure the reflectance spectrum curve, and 9 vegetation indices including PRI, mSR705, mND705, R470/R570, R520/R675 were calculated. These vegetation indices are closely related to the photosynthetic capacity, steady-state fluorescence, chlorophyll concentration, etc. of rice leaves/panicles (Gil Ortiz R et al. 2020).

Figure 1. Time trend of effective quantum yield QY of different rice varieties

Figure 2. Linear regression coefficients between reflectance vegetation index and chlorophyll fluorescence parameters

reference

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