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Desktop ATP fluorescence detector

Pi-102 Food Bacterial Rapid Detection Device ATP, short for Adenosine Triphosphate, is an intracellular energy supplying molecule. The Pi-102 desktop

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[Product Name]Pi-102 Food Bacterial Rapid Detector
[Product Model]Pi-102
[Product Parameters]handheld
[Product Introduction]
Pi-102 Food Bacterial Rapid Detector (Desktop ATP Fluorescence Detector)

ATP, short for adenosine triphosphate, is an intracellular energy supplying molecule. Pi-102Food bacteria rapid detector detects bacteria in samplesATPThe luminescence reaction with luciferase yields a light unit reading, which can be converted to estimate the total ATP level in the sample, further indirectly determining the total number of microorganisms in the sample and providing reference for on-site testing of food hygiene supervision.

Firstly, use relevant reagents to degrade and eliminate ATP outside the bacteria. Reusing the extraction solution to release bacteriaATPAnd prevented by enzymesATPAfter degradation, add luciferase and ATP for luminescence reaction.

The luminescence value measured by a high-sensitivity photomultiplier tube is proportional to the actual ATP present. By comparing with the standard sample, it is possible to calculate the value of the tested sampleATPContent andfgOr expressed in RLU form.

[Scope of Application]
Hygiene monitoring in the food industry, cosmetics manufacturing industry, and medical institutions.

Combined with specialized reagents, it can quickly evaluate the total number of microorganisms in liquids, solid foods, surfaces, etc. Note that the results are for reference only.

[Configuration Status]

1 fluorescence detector host

Testing reagent (optional) Food bacterial total detection kit

[Technical Parameters]

Detection accuracy 1 X 10^-18mol/ATP/assay

Default detection time 5 second(Adjustable from 0.2 to 100 seconds)

Detection mode RLUfg(ATP content in bacterial cells)

The data storage results can be classified and stored, with 800 memories stored

Screen display LCD

Size208 *105 * 93(Long)*wide*tallmm） / 1.5 kg

Measurement value 0-99999 RLU

**Error+/-5%

Real time detection and transmission of results when connected to a PC

Built in Ni/Cd rechargeable battery for power supply, external power source

[Product Comparison]
Comparison between Pi-102 Bacterial Rapid Analyzer and SystemSURE Plus ATP Fluorescence Analyzer

	Pi-102 Food Bacterial Rapid Analyzer	SystemSURE Plus ATP Fluorescence Analyzer	compare
reaction mechanism	ATP bioluminescence detection	ATP bioluminescence detection	same
Instrument volume	Small desktop	handheld
sensitivity	10 -17 –10 -18 mol ATP	10 -15 mol ATP Pi-102	Nearly 1000 times higher
Supporting photoelectric cell	High sensitivity photomultiplier tube	Ordinary photomultiplier tube
Test Item	Can detect liquids, solid foods, and surfaces	surface	Pi-102 has a wider range
Use reagents	Rapid detection kit for total microbial count in food Water quality ATP detection kit Ultrasnap TM Integrated Standard Reagent Aquasnap TM Integrated Water Sample Reagent Aquasnap TM Integrated Water Sample Reagent	Ultrasnap TM Integrated Standard Reagent Aquasnap TM Integrated Water Sample Reagent
Upgrade scalability	Can be used in conjunction with magnetic beads for further qualitative detection of pathogenic bacteria
Critical value setting	High risk control 5000-18000 RLU Conventional control above 18000 RLU	High risk control 10-30 RLU Conventional control 30-100 RLU
review	The Pi-102 food bacteria rapid analyzer and SystemSURE II ATP fluorescence analyzer are both products under the Hygiena company. Both are designed to detect different items. The SystemSURE II ATP fluorescence analyzer is small and portable, but has low detection sensitivity and is suitable for rapid surface detection on site. The Pi-102 food bacteria rapid analyzer is relatively more precise and complex, equipped with high-sensitivity photomultiplier tubes and reaction kits. It can not only detect surfaces, but also detect microbial levels in liquid and solid foods (pre treated).

【 Related Certificates 】

Pi-102 Food Bacterial Rapid Detector and SystemSUREⅡATPFluorometerSame as products under the Hygiena company. Both are designed to detect different items,

The SystemSURE II ATP fluorescence analyzer is small and portable, but has low detection sensitivity, making it suitable for rapid surface detection on site.

Pi-102 Food Bacterial Rapid Detector is relatively more precise and complex, equipped with high-sensitivity photomultiplier tubes and reaction reagents

The box can not only detect the surface, but also detect the microbial level in liquid and solid food (pre treated).

American MPC（Medical Packaging Corporation）Head office——Hygiena's parent company issues:

US Department of Public Health and Social Welfare (HHS)

Food and Drug Administration License No. 2023790

California Department of Public Health, USA

Food and Drug Administration Production License No.: 61193

Note: This product does not belong to medical devices

catalogue

1. Instruments

1.1 Instrument Introduction

1.2 Application of Test Tube Size

1.3 Key operation instructions

1.4 Power Management

1．5Install snapshot detection warehouse

2. Measurement

2.1 Quick Start

2.2 Measurement Options

2．2. 1 Table of Contents

2．2. 2 Options

3. Maintenance and troubleshooting

3.1 Maintenance

3.2 Troubleshooting

Warranty Commitment: Commitment Statement

[User Manual]
Pi-102 Food Bacterial Rapid DetectorFluorescent Instrument User Manual

Version: 1.0

Hygiena International Ltd.

June 2002

Hygiena guarantees in Pi-102Food Bacterial Rapid Detection DeviceAfter being sold12 Responsible for its performance and functionality within a month. The impact caused by appropriate operationsDamage of Pi-102 Food Bacterial Rapid Detector

The use of unauthorized spare parts is not covered by this warranty.

Warning:

Please read this manual carefully before using the Pi-102 Food Bacterial Rapid Detection Fluorescence Analyzer.

Please make sure to disconnect the power before cleaning. Only use a damp cloth to clean the surface of the instrument, any cleaning agent may damage the surface of the instrument.

Only Pi-102 Food Bacterial Rapid Detector can be usedSpecial power plug, other power devices may damage Pi-102.

Only authorized and trained personnel are allowed to engage in the repair and maintenance of the Pi-102 food bacterial rapid detector.

This manual provides detailed descriptions, and Hygiena is not responsible for any damage caused by improper use.

All rights related to this document belong to Hygiena, and no content of this document may be reproduced without written authorization from Hygiena.

Hygiena International Ltd. 和 Hygiena LLCIt is a subsidiary of Medical Packaging Corporation Inc. in the United States.

1. Instruments

1.1 Instrument Introduction

The Pi-102 Food Bacterial Rapid Detection Fluorescence Analyzer is a highly sensitive instrument for measuring bioluminescence and chemiluminescence. It can be used independently or in conjunction with a PC and supporting software

to be connected.

1.2 Application Test Tube Specifications

The standard size of a Hygiena test tube is12×55mmBut the diameter is11－12mm, high in47－Test tubes within a range of 82mm can be tested on Pi-

Used on machine 102.

The Pi-102 Food Bacterial Rapid Detector also provides an additional compartment for using Hygiena snapshot swabs for hygiene monitoring. In this case, there is no need to use

Test tube.

1.3 Keyboard and Display

ON/OFF: Turn on key

◄◄: Return to the upper menu or use the escape key

◄►: Browse menus

Select the program from the menu or press the enter key

O O O: Display results

Regarding Pi-102

Supply: Connect the power supply

Charge (Std.): Use power to operate Pi-102

Charge (Fast): A fast charging device

1.4 Power Supply

Users can choose to use a power source or a rechargeable battery. When using the power supply, Pi-102 food bacteria rapid detectorCharge the battery.Pi-102Food Bacterial Rapid Detection DeviceOn one side“Charge”

The selection key should be placed in "Std”Location. If using fast charging, after plugging in the power, it will“Charge”The selection key“Fast”The battery model is Ni/Cd,

To avoid 'memory impairment'”Okay, wait“Empty "indicates to wait for the light to turn on before charging.

1．5Install an additional warehouse for snapshot detection

Suggest turning on the Pi-102 food bacteria rapid detection devicePreviously installed. Just be careful to remove itPlace the lid on the Pi-102 food bacterial rapid detection device, attach the additional part, and then install the lid on the additional part

At the top, the additional compartment is easily installed. During installation, pay attention to the correct connection of each component and install it in the correct position. In this way, the Pi-102 food bacteria rapid detector can detect

The snapshot device has been installed. To remove the additional compartment, simply remove the compartment cover and additional compartment, and then cover the compartment cover back to the compartment opening.

2. Measurement

2.1 Quick Start

▪ Push the ON/OFF button

▪ Press (directory)

▪ press （OK）, or press►Select 02... 08 from the directory and then press

▪ Press (new), the old results in the directory are**

▪ Start measuring

▪ Next specimen, press twice

▪ The results have been saved. Press ◄ to browse the previously saved results in the directory

▪ Press on both sides to push out the measurement

▪ pressON/OFFPress the key twice to close Pi-102.

Attention: Please keep the measurement chamber closed during the measurement process and remove the specimen after the measurement is completed.

2.2 Measurement Options

After booting up, the screen displays two lines of text: LIST (directory) and OPTIONS (options).

2．2. 1 Catalog:

You can choose 8 different directories, each of which can perform 100 tests.

Please press to select the directory, and the screen will display:

List 01

Name ……

100 pos , 00 used

Esc < > Ok

Choose one of the following four possibilities:

▪ Press ◄◄ to return to the home directory

▪ Press ◄ to select the directory with low digits

▪ Press ► to select the directory with high numbers

▪ Confirm to display directory

After pressing, you will see the following display:

Old results from

……………(date + time)

100 pos

Esc Old New

Choose one of the following three possibilities:

▪ Press ◄◄ to return to the home directory

▪ press►View and mark old results in the selected directory. If there are no old results,Pi-102 Food Bacterial Rapid Detector will require you to insert * * specimens

▪ press

Delete old results from the selected directory

After pressing, you will see the following display:

…. .(List Name) / 1

Name 1

Insert Sample

No Thresholds

Insert the specimen and press

Start the inspection, and the green, yellow, and red indicator lights will light up during the inspection process. The detection is complete, and you will see the following display:

…(List Name) / 1

Name 1

2 RLU

No Thresholds

Start the next test, press twice. If your previous result record is too long, please press

Press again. After completing the measurement, press ◄◄ twice. Press the ON/OFF button twice more to turn off the device.

Then select 'Overwrite result'”Press the ► key

You can select the 'Options' directory from the home directory. In this directory, you can choose to change the settings of the fluorescence analyzer. Here is an explanation of these settings:

Select the option from the home directory”Just press the ► key and then press again

Units

Counting time

Self Test

Print List

The display is as follows:

Units: Results can be selected in RLU formatorfg ATPexpression. Generally speaking, detection needs to be set up using RLU.

Counting time: The default setting requires 5.0Seconds, programming in0.2－100.0 Within seconds, use ► Press the key again to program. The difference in counting time will not causeRLUThe change in value is due to the displayedRLUThe value isn0.2 seconds of time

The average value of the interval. The longer the counting time, the more accurate the RLU value.

Self Test: This is to check whether the measuring chamber is avoiding light. The Pi-102 Food Bacterial Rapid Detector will perform a blank test and return to the catalog. Before starting self inspection, please check if the measuring chamber is vacant.

Hygiena PI-102 Food Bacterial Rapid Detector

Microbial ATP Kit HS Biothema 266-112

1. The reagent kit includes the following reagents: (Each set of reagents can complete 100 experiments)

1.1. ATP Reagent HS (4 vials)Contains freeze-dried luciferase and luciferin. After participating in the reaction, luciferase consumes approximately 6% ATP per minute.

1.2. Diluent B 10ml (4 small bottles):Used as a buffer for reconfiguring ATP ReagentHS.

1.3. Extractant B/S 10 mL (1 small bottle): extractant.

1.4. ATP standard 5 mL (1 vial)：ATPStandard solution, containing 10^-7mol/L ATP。

1.5. ATP Eliminating Reagent (1 小瓶)ATP eliminator.

1.6. Cell Lysing Reagent 10 mL (1 小瓶)Cell lysis agent, used for reconstitutionATPATP Eliminating Agent

Reagent）

2. Detection principle:

2.1. All living cells contain adenosine triphosphate (ATP)（Adenosine TriphosphateMolecular formulaC₁₀H₁₆N₅O₁₃P₃Chemical formulaC10H8N4O2NH2(OH)2(PO3H)3H, with a molecular weight of approximately 507.184)

2.2. Inside a bacterial bodyATPThe content is approximately2x10^-18mol,After cell death,ATPUsually, it also disappears along with it. By detecting the ATP content in the sample, the total number of bacteria in the sample can be calculated.

3. Preparation before using reagents:

3.1. Uncover (do not use hands directly) ATP Reagent HSbottle cap. uncoverDiluent BBottle cap, willDiluent B 倒入 ATP Reagent

HS reagent bottle, mix and then pour the mixture back into Diluent Breagent bottleStore in the dark and refrigerated.

3.2. Extractant B/S and ATP Standard reagents can be used directly.

3.3. Mix ATP Eliminating Reagent with Cell Lysing Reagent for use. The mixture can be stored in a refrigerated state for more than a week,

Store in a frozen state for a longer period of time.

4. Detection reaction steps:

Firstly, ATP outside the bacteria is degraded by enzymes. Then useExtractant B/SRelease intracellular substancesATPAnd prevent the decrease of ATP through enzymes

Solution. *Then add ATP Reagent HS to analyze ATP.

The ATP measured by a photometer and the actual presentATPIt is proportional. By measuring and comparing before and after adding a certain amount of standard ATP

The result may be used to calculate the ATP content of the unknown sample being testedfgOr expressed in RLU form.

5. Testing steps:

1) Take the tested sample for pre-treatment according to international methods. (As explained later)

2）Take processed samples50µLSamples and50µL ATP Eliminating ReagentLet it stand in the test tube for 10 minutes.

3）Join directly50µL Extractant B/SIn the test tube, confirm that all ATP Eliminating Reagents have been tested

Extractant B/S underwent a reaction (using a vortex mixer or manually shaking for a few seconds).

4）join0.4mL ATP Reagent HSAnd measure M1 Ismp.

5）join10µL 100 nmol/L ATP Standard, about1pmol（10^-12）ATPMeasure its value again, M2 - Ismp+std。

Attention: M2- M1：M1The value should be greater than 10, otherwise the sample needs to be diluted again for testing.

6）Calculate the sample using this formulaTotal ATP count.

The formula is: M1

M2 - M1 = X

7）Evaluate the test results and calculate the bacterial count of 1ml of the sample.

The formula is: X * 10⁷

The result value is an approximate value and is recommended for reference.

National Food Microbial Limit Standards (Partial)

Types of food

grilled meat

Sauce braised meat

Please refer to the relevant standards for details.

ex-factory

sales

ex-factory

sales

Total colony count [cfu/g (ml)]

<5000

<50000

<30000

80000

6. Attention:

The test tubes and plates used in the experiment must ensure the absence of ATP.

2. Please use opaque glass or plastic containers to store fluorescent reagents, and be careful to avoid strong light exposure during the reaction.

3. A culture medium containing bacterial suspension can be used as a blank control.

4. For the same sample, it is recommended to conduct three sets of repeated experiments.

5. Samples added to the experimentATP Eliminating Reagent、 The extractant B/S quantity must be consistent.

The difference in the ratio of Extractant B/S and ATP Reagent HS shall not exceed 1/4

7. The standard dosage of ATP is always 10 μ L

The more reagents used in the experiment, the higher the accuracy of the results.

9.（Ismp+std^{- I}SMP）Value should be comparedI_SMPIf the value is more than 10 times higher, the sample needs to be diluted again.

10. If the sample is non bacterial ATPThe content is relatively low, and the step of non bacterial ATP can be omitted.

If there is no extracellular free ATP in the sampleIt is recommended to use the 266-311 reagent kit.

The effect of extracellular free ATP may still be significant in the following situations:

Excessive extracellular free ATP content in A cells

The detection rate of ATP in B cells is too low

The medium in which the C sample is located has an inhibitory effect on the ATP degrading enzyme reaction

Corresponding to the above situation, the incubation time can be extended by 10 minutes or more concentrated ATP Eliminating Reagent can be added

[Overview]
Pi-102 Food Bacterial Rapid Detector uses ATP bioluminescence method, combined with high-sensitivity photomultiplier tubes and specialized reagents, to quickly estimate the total number of microorganisms in food samples (pre treated) within 10 minutes. The result values correspond to the corresponding national standards, which can preliminarily determine the microbial situation in food samples, such as whether they exceed the standard.

However, the results obtained by this method are for reference only. Due to the influence of various factors (temperature, acidity, dilution concentration, etc.) on ATP bioluminescence reaction, there is a certain deviation between the results and the CFU (colony forming unit) obtained by conventional culture counting. Therefore, it is recommended that the customer set a critical value for reference in this test.

Compared to conventional culture counting methods, this detection is fast and sensitive, especially when detecting samples with low microbial content such as water samples.

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Regarding Fiteli
Shenzhen Fiteli Technology Co., LtdMain agency products:

Equipment for sample testing：

♦RBG's RVLM Microbial Rapid Detection System and RVL Rapid Detection Bottle from Germany
(Total number of live bacteria, coliforms, Escherichia coli, Enterobacteriaceae, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella, Listeria monocytogenes, Enterococcus, Clostridium sulfite reducing Clostridium, Clostridium perfringens, Aspergillus, Aspergillus, yeast, etc.)
♦ Swedish Meris MIRIS mid infrared dairy analyzer;
♦ Rapid detection instrument for total bacterial count in raw milk;
♦ Bostwick viscosity meter and moisture analyzer from CSC Corporation in the United States;
♦ Double U-shaped resonant intelligent liquid density meter/concentration meter/alcohol density meter;
♦ GoodJob series gel imaging products;
♦ Astori freezing point analyzer and colony counter from Italy;
♦ Organomation nitrogen blower from the United States;
♦ Japanese ATAGO refractometer/polarimeter;
♦ Palintest water quality analyzer from the UK;
♦ National Brick Products: Milk Microbial Activity Rapid Monitor, Grain Microbial Activity Rapid Monitor;
♦ Food sulfur analyzer (rapid detection of sulfur dioxide, sulfite, and suspended white blocks)
♦ Hygiena series products from the United States: SystemSURE Plus ATP fluorescence analyzer, EnSURE multifunctional ATP fluorescence analyzer
♦ And various food industry related testing instruments and consumables

Equipment used for sample processing:

♦ German ELMA ultrasonic cleaning machine;
♦ German KERN balance;
♦ ADAM balance in the UK
♦ Endecots screening machine and sieve from the UK
♦ Efficient tapping homogenizer/high-pressure homogenizer/ultra clean workbench/bacterial incubator from South Korean BNF company;
♦ SONICS ultrasonic disruptor from the United States.
♦ CENTURAON centrifuge from the UK;
♦ Italian PBI microbial sampler;
♦ AES Air Microbial Sampler, Beating Homogenizer, Automatic Dilution Device, and Culture Medium Automatic Preparation and Packaging System from France;

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Microbial Rapid Detection System
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Ultrasonic cleaner
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