Biodisruptor Pico second-generation sequencing and ChIP experimental sample pretreatment ultrasonic disruptor

Bioruptor Pico.pdf

Technical parameters:

Ø Control mode:

-Ultrasonic sample volume: With different adapters, the volume of ultrasonic samples can reach 2ml or more or 5ul or less

-Applicable sample types: Applicable sample types include 0.1ml, 0.65ml, 1.5ml, and 15ml centrifuge tubes, which do not require the use of special materials (such as glass) consumables, saving experimental costs

-The sample needs to be crushed in a closed container without producing infectious flying mist, and no additional ultrasonic probe needs to be inserted

-Allowable single operation quantity: up to 12 samples can be processed at a time (requires 0.1 or 0.65ml adapter)

-The sample needs to be able to automatically rotate at a constant speed during ultrasound to ensure consistent crushing effect

Ø Instrument lifespan monitoring device

Ø Instrument overheat protection device

Ø Sample crushing method: Ultrasonic crushing is required

Ø Nucleic acid sample fragmentation size: 1kb~100bp or smaller

Ø The instrument includes a set of 0.65ml adapters: with a knob type 0.65ml centrifuge tube sample explosion-proof and leak proof safety fixing lock, at least 12 0.65ml tubes need to be used simultaneously. The reaction volume of a single sample can range from 10 µ l to 100 µ l

Ø Ultrasonic power/time adjustment accessories:

Ultrasonic on/off dual timer: can automatically control the number of ultrasonic on/off cycles (number of cycles), with a timer range of 1-99 cycles

Multi functional timer: Sample ultrasound start timer: can be set within the range of 1-99 seconds.

Ø Sample ultrasound pause timer: can be set within the range of 1-99 seconds

Ø Automatic shutdown protection device for instrument overheating, monitoring device for instrument usage status, and self state detection device for startup operation

Equipped with a cooling circulation system and solenoid valve, it can be linked with the ultrasonic host. When the ultrasonic is started, the cooling system pauses the circulation. When the ultrasonic is paused, the cooling system starts without interfering with the ultrasonic efficiency

Equipped with a pause function, the anti noise upper cover is lifted, and the ultrasonic instrument pauses immediately. After closing the cover, ultrasound continues, which can accurately grasp the sample ultrasound time and increase the safety of experimental personnel operating the instrument

Ø Configuration includes: integrated ultrasonic power/time adjustment host ultrasonic water tank and anti noise upper cover, adapter rotating upper cover, 0.65ml adapter and solenoid valve cooling circulation machine

Main functions:
1. DNA fragmentation of second-generation sequencing samples
2. Immunoprecipitation experiment