Product Introduction:

Sudan Red is an artificially synthesized industrial dye that is carcinogenic. Therefore, it is necessary to strictly monitor whether the feed contains Sudan Red dye. our companyTengzhou Hongxiang Analytical InstrumentRefer to agricultural industry standards（NY/T 1258-2007）By utilizing a new high-performance liquid chromatography instrument, it has been tested through practical testing to provide information on the presence of Sudan Red dye in feedHPLCThe detection plan provides accurate and reliable results with good detection limits, suitable for Sudan Red in compound feed, concentrated feed, and additive premixed feedⅠSudan RedⅡSudan RedⅢSudan RedⅣThe measurement.

Detection principle
The Sudan Red dye in the sample was extracted by shaking with acetonitrile, dried and concentrated under nitrogen, and then injected into the reverse phase chromatography system of a high-performance liquid chromatograph for analysis
Preparation of Sample Solution
extract
Weigh appropriate amounts of sample mixed feed, concentrated feed, and additive premixed feed separately, place them in a stoppered conical flask, add acetonitrile, shake and extract in an oscillator, centrifuge, and set aside the supernatant.
Accurately extract an appropriate amount of feed extract from the test solution, blow dry with nitrogen, add acetonitrile, mix with a turbine mixer, and pass through a pore size of0.45μmOrganic microporous filter membrane, filtrate analyzed on the machine.
Concentrated feed and pre mixed feed extract with additives are directly analyzed by high-performance liquid chromatography.
Chromatographic conditions Chromatographic column:VertexChromatographic column250mm×4.6mm×5μm；
Mobile phase: Acetonitrile+Water, gradient elution;
Current Speed:1.0mL/min；
Temperature: room temperature;
Injection volume:50μL；
Detection wavelength: Sudan RedⅠfor478nmSudan RedⅡSudan RedⅢSudan RedⅣBoth are520nm。