This is a minimal essential culture medium with added nutrients, containing all 21 normal amino acids and 5 additional vitamins. Minimum Essential Medium (MEM) is a common cell culture medium developed from Eagle Basic Medium (BME). It contains high concentrations of amino acids, which are closer to the protein composition of mammalian cells. After adding serum, MEM can be used to cultivate various monolayer growing cells, such as fibroblasts.

Modified Minimum Essential Medium (MEM) for alpha modification