2020 edition of the Chinese Pharmacopoeia General Rule 1101 Sterility Test Method

Take the specified amount of sterile aerosol test sample and transfer it to a closed membrane filter using specialized equipment. Or freeze each container at -20 ° C or other suitable temperature for about 1 hour, remove it, quickly disinfect the opening part or valve of the test sample, place the container upright, and use sterile steel cones or needle sampling equipment to quickly drill a small hole in a suitable position that matches the structure of the container valve through aseptic operation

General Rule 1105 of the 2020 edition of the Chinese Pharmacopoeia: Microbial Limit Test for Non Sterile Products: Microbial Counting Method

Take the aerosol test sample, freeze it at -20 ° C or other suitable temperature for about 1 hour, remove it, and quickly disinfect the opening part or valve of the test sample. Place the container upright and drill a small hole in a suitable position that matches the valve structure using a sterile steel cone or needle sampling device